Microsatellite stutter bands

Nick Jacobsen jacobsen at cf.ac.uk
Fri May 30 08:31:37 EST 1997


Hi there,
        We do an large amount of microsatellite PCR in my lab. The 
presence of stutter bands doesnt usually hinder accurate genotyping of 
samples. We dont make efforts to get rid of them. Par for the course I 
think unless you want to try a fancy proof reading enzyme instead of Taq 
although we have tried this with fluorescent DNA pooling and it doesnt 
seem to make much difference

cheers

Nick 




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