Differential Behaviour of DNA upon Untwisting (Article and link)

Graham Dellaire dellaire at odyssee.net
Tue Jan 28 09:40:13 EST 1997


For full article see

 http://www.medcor.mcgill.ca/EXPMED/ivan/article1.html
 
Proc. Natl. Acad. Sci. USA
Vol. 94, pp. 403-406, January 1997
Biochemistry
 
 
Ivan Brukner, Abdellah Belmaaza, and Pierre Chartrand* 
 
Institut du Cancer de Montreal, Centre de Recherche Louis-Charles Simard,
1560, rue
Sherbrooke Est, Montreal, PQ Canada H2L 4M1
 
Communicated by Richard E. Dickerson, University of California, Los
Angeles, CA,
November 25, 1996 (received for review April 30, 1996) 
 
 
ABSTRACT
 
We have synthesized DNA segments with different handedness, twisting and
radii of curvature, and have analyzed the effect of untwisting on them. The
results
indicate that the dynamic behavior of curved DNA upon untwisting is
strongly determined by the initial sequence-dependent DNA trajectory. In
particular,
DNA with the same radii but with opposite handedness of superhelix twisting
can
show very different conformational responses to ethidium bromide
untwisting.
Upon treatment with ethidium bromide, right-handed superhelixes decrease
their
twist and increase the planarity of the superhelix, while left-handed
superhelixes increase twisting and decrease their degree of planarity. 

 
Posted for Dr. Ivan Brukner
 
brukneri at ere.umontreal.ca



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