5'Ends/GC-rich mRNA/Rare and Alternative

[Carla Wood]

I am investigating the RACE/5'-end technologies for
rare GC-rich mRNA
templates with multiple  5'-ends/promoters. I have
used the following kits:
Marathon and SMART RACE kits (Clontech) and 
Sure-RACE cDNA panels (OriGene
Tech., tissue panels), with limited success to date.
I am now
turning to the Version 2.0 5'-RACE kit from
Gibco/BRL, as it has been
improved slightly. Also I am
looking into any techniques or kits (kits, not
developed yet?) for
ligation-mediated RT-PCR/5'-RACE
which can be useful for rare messages/alternative
5'-ends.
Thanks for any suggestions anyone has found.
C. Wood
 
> _______________________________________
> 
> Carla M. Wood, Ph.D.
> Parker Hughes Cancer Center, The Hughes Institute
> 2657 Patton Rd.
> St. Paul, MN  55113
> 
> Phone:  651/628-9988 ext. 206
> 
> E-mail: cwood at mercury.ih.org
> 
> 
> 

===



Moderated
bionet.genome.gene-structure


_____________________________________________________________
Do You Yahoo!?
Free instant messaging and more at http://messenger.yahoo.com