The large scale mutagenesis project

JanCzek janczek at aol.com
Tue Mar 21 17:50:12 EST 2000


The Jackson Laboratory launches large-scale
mutagenesis project.
  The Jackson Laboratory (TJL) has recently launched a
program to
systematically
collect a large number of mouse mutants to provide new
models relevant 
to
the study of human neurological diseases.  TJL’s
Neuroscience 
Mutagenesis
Facility will generate, identify and characterize new
mutants in a 
broad
range areas including motor function, epilepsy,
neural-based obesity,
hearing, vision, learning, ingestive behaviors,
affective disorders,
sensorimotor gating, substance abuse and anxiety.
As part of this program, supervisor Dr. Kevin Seburn,
approached 
Columbus
Instruments in February, 1999 to develop an automated
monitoring system 
for
use in the initial detection of deviant mice.  The
result of this
collaboration was a state-of-the-art live-in cage
(dubbed “CCMS” or
Comprehensive Cage Monitoring System) that allows
24-hour, automated,
non-invasive collection of several physiological and
behavioral 
parameters
simultaneously (activity, food and water consumption,
metabolic
performance).  The proposal for the development of
these cages as an
automated screening tool was based on the simple
premise that the 
detection
of aberrations in any complex system is best achieved
by simultaneously
examining several parameters. To test this notion a
variety of known 
but
subtle, non-visible mutant mice were placed in the
monitoring cages.  
The
data were then compared to controls using a specially
developed 
statistical
algorithm that exploited the multiple measures
provided by CCMS.  
Initial
results were very positive and showed that at least
six different types 
of
mutants were successfully detected using CCMS data. 
The use of CCMS as 
a
tool for initial mutant detection in combination with
more focused 
secondary
screens promises to be an efficient means of
characterizing important 
new
mouse models for study of neurological disease.
The Jackson Laboratory, founded in 1929, is a world
leader in mammalian
genetics research. With approximately 1,000 employees,
the nonprofit,
independent facility has a mission to improve the
quality of human life
through discoveries arising from its own genetic
research and by 
enabling
the research and education of others.  Further
information on The 
Jackson
Laboratory can be obtained at http://www.jax.org.
Information on Columbus Instruments equipment used in
this project is
available at: http://www.colinst.com
Jan Czekajewski, Ph.D.
janczek at aol.com


=====



Moderated
bionet.genome.gene-structure



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