phosphorylation

[Pietro Pichierri]

I would like to know whether someone could help me
about the use of anti-phosphoserine antibodies.
I am using anti-phosphoserine kit from calbiochem
(different monoclonal antibodies against different
motifs containing pSer) but with little or not
results.
I cannot use 32P labelling because I am studying
radiation-induced protein phosphorylation.
I have high backgrounds on blots, despite I use BSA
instead of NFDM, an poor results.
Could someone suggest me other antibodies or a
well-working protocol?
Thanks a lot in advance

Pietro
-------------------------------------------------
Dr. Pietro Pichierri, PhD
UPR2169 CNRS "Genetic Instability and Cancer"
Institut de Recherches sur le Cancer, Andrè Lwoff
7, Rue Guy Moquet - B.P.8
94801 Villejuif - France

Phone: +33 (0)1 49583409
Fax:      +33 (0)1 49583411
 

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Moderated
bionet.genome.gene-structure



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