subcloning

[richard Shen]

I have a BAC clone in which the insert is around 160
kb (genomic DNA).
I want to subclone it into proper vector. According
to the routine
way, i.e. to purify the inserts one by one then
ligate them into
dephosphorylated vector respectively, it will be
very time and labor
consumimg. Is there any other smart way to solve the
problem?
Thank you in advance!
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Moderated
bionet.genome.gene-structure



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