Deglycosylation of fetuin

Steven Pirie-Shepherd srps at galactose.mc.duke.edu
Mon Apr 3 20:34:16 EST 1995


Stefan.Rosen at mbioekol.lu.se wrote:
: Hello!

: I want to enzymatically pile off the O-linked sugars (totally) in fetuin
: (from fetal calf serum). Are there anyone that have own practical
: experience about this subject and knows which enzyme(s) that works best
: (company,no, extent of deglycosylation..) ?. 

: Thank you,



There is a company in Novata (o?) California, called Glyko. They have an 
interesting range of recombinant glycosidases.

1. An O glycosidase that will cleave the Gal GalNAc moiety from the 
Ser/Thr...HOWEVER, all substituions must be removed first, this can be 
achieved using sialidases, Glyko sell one which will cleave all three 
linkages (2-3, 2-6, 2-8) in combination with fucases, thus either a 
cocktail approach or sequential degradation is required. Since most 
O-linked sugars are much simpler in structure that N-linked, a cocktail 
of 1 to 3 enzymes should do the trick. Other companies whjo might have 
such enzymes are Boehringer (although they recently stopped selling 
ConA..which I found a starnge marketing move?!*), and Oxford glyco 
systems..on no account buy enzymes from Sigma, they are usually sourced 
from elsewhere. If anyone is really desperate I can put the phone number 
of GLYKO onto this newsgroup..pending thier approval, BTW I am in no way 
related to GLYKO, I just like their stuff, as it is good gear.

__________________________________________________
Steven Pirie-Shepherd		       		
srps at galactose.mc.duke.edu	        
"Insert your own pithy phrase just about here!"



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