Enzyme Kinetics-Hehre Mechanism

Roger Graham flo at unixg.ubc.ca
Sun Apr 9 19:21:21 EST 1995


I was wondering if there is anyone who is familiar with hydrolysis by
glycosidases of glycosyl fluorides having the wrong anomeric
configuration(Hehre mechanism).  With this type of mechanism, hydrolysis is
substrate activated and two molecules of substrate must bind in the active
site first.  Plots of RAte vs substrate concentration and 1/rate vs
1/substrate give upwardly concave curves.  

What I would like to know is what is the equation to describe these types
of kinetics and if possible, how is it derived.  A reference would be very
helpful also. Please mail me directly.
Thanks 
Howard
howie at unixg.ubc.ca



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