Endoglycosidase

[Ralph Melcher]

I am searching for an enzyme (or enzymes) with a specific cleavage side for 
the ß-1-6-bound N-Acetylglucosaminyl residues (linked to the mannosyl core of 
N-linked oligosaccharides), which defines tri- or tetraantennary complex 
N-linked oligosaccharide-types. By the use of this type of enzyme-activity I 
hope to be able to discriminate between N-linked complex biantennary and 
tri/tetraantennary oligosaccharides respectively.

Beyont that, I want to know if there exist any inhibitor of glycoprotein 
biosynthesis which has the property of specifically inhibition of the synthesis
of the ß-1-6-antennae or the ß 1-4-antennae of N-linked complex 
oligosaccharides.


Many thanks