2D PAS

[Aida Cancel]

I have been trying to use PAS staining to detect glycoproteins in 10-17.5% 
(1.5 mm) 2D SDS-PAGE gels of seminal plasma proteins...needless to say, 
without success.  I have tried 4 different protocols (including doing the 
staining after transfer of the proteins to nitrocellulose).  My problem is 
that there is little or no color development.  I am loading lots and lots of 
protein...and for a fact I know that many of these are extensively modified 
and have carbohydrates attached.  Can anyone help me?  Any recommendations?

(Please send reply to axc19 at psu.edu)

Thanks,
Aida