I have been trying to use PAS staining to detect glycoproteins in 10-17.5%
(1.5 mm) 2D SDS-PAGE gels of seminal plasma proteins...needless to say,
without success. I have tried 4 different protocols (including doing the
staining after transfer of the proteins to nitrocellulose). My problem is
that there is little or no color development. I am loading lots and lots of
protein...and for a fact I know that many of these are extensively modified
and have carbohydrates attached. Can anyone help me? Any recommendations?
(Please send reply to axc19 at psu.edu)
Thanks,
Aida
