monosaccharide analysis

Patricia_W._Cash_at_~PRISYR05 at ccmail.bms.com Patricia_W._Cash_at_~PRISYR05 at ccmail.bms.com
Tue Jun 13 13:51:19 EST 1995


     Dear Chisoon Chang:
     
     You wrote that you were worried about amino acids sticking to the 
     PA-1 column. I don't believe that the amino acids stay on the column.  
     I know that some amino acids interfere with quantitation of sugars 
     (i.e. lysine elutes very close to galactosamine).  Does any one else 
     have information about amino acids sticking to the PAC-1 column?
     
     I use a 2N TFA hydrolysis for neutral monosaccharides and 6N HCl 
     hydrolysis at 100 C for 4 hours for the amino monosaccharides.  I am 
     primarily analyzing glycoproteins.  Glycopeptides would probably have 
     more amino acid interference. 
     
     Please contact me if you want more details.  My email is cash at bms.com
     
     Thanks,
     Pat Cash




More information about the Glycosci mailing list