O-linked glycosylation

Tim Dudgeon dudgeon at britbio.co.uk
Fri Mar 17 10:19:14 EST 1995


I have produced a recombinant protein in the yeast Pichia pastoris that is
partially glycosylated with O-liked sugar (there are no N-linked sites in the
protein). I wish to seperate the glycosylated form the unglycosylated protein.
I have tried using ConA-Sepharose (Pharmacia) and Glycine-Max (Sigma) without
success (neither seemed to bind the glycoprotein). The ConA came with
instructions, but I have not been able to dig out any protocol for using the
Glucine-Max and Sigma were not forthcoming.

Has anyone has any experience in using these lectins, or knowledge of any other
techniques that might be of use, I would like to hear from you.

Please reply to my email address as I don't regularly read this newsgroup.
Apologies also to those who frequent the  protein-analysis newsgroup as this
message was posted there first, before I became aware of this more appropriate
forum

Many thanks.

Tim


-- 
Tim Dudgeon
British Biotech
Phone: 0865 748747
FAX:   0865 717598
email: dudgeon at britbio.co.uk




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