electroeluted protein

John Proudman, GGPL, B-262 johnp at GGPL.ARSUSDA.GOV
Fri Mar 24 15:03:50 EST 1995

Hi, Aida:

Electroelution (or any other electrophoretic procedure) should not affect the 
detection of glycosylation by the Oxford kit.  Electrophoretic procedures are 
routinely used in the purification/separation of glycosylated proteins.


>On 24-Mar-95 14:13  Aida Cancel {axc19 at psu.edu}  wrote:
>HI!  Can anyone tell me if using a protein that has been electroeluted to
>detect possible glycosylation of the protein is accepted for publication?
>In other words: I purified a protein by electroelution and I am
>interested in knowing if it is glycosylated.  I bought a glycosylation
>detecting kit (Oxford) but I would like to know if using electroelution
>will harm glycosylation of the protein to the extent that it can not be

More information about the Glycosci mailing list