protein standards

rao thotakura rao_thotakura at HGSI.COM
Wed May 17 15:53:47 EST 1995


Regarding the glycoprotein standards to be used, a positive control would be 
the popular bovine fetuin or transferrin.

A negative control is a tough one as seen in the discussions.
After considering recombinant proteins, I found Affinity purified BSA (Pierce, 
cat no. 30444=6x10mg or 30443=1g). I used this as a carrier during 
deglycosylation reactions and this has given me virtually no background when 
analyses were done by HPAE-PAD.

Please let me know how it works for you.

Rao.




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