measuring glucose and trehalose

Dr Koen De Smet kds30
Fri Oct 20 06:52:02 EST 1995


I would like to measure trehalose in samples with an expected 
concentration of 10-50 ug/ml (25-125 uM). One way is by using 
hot anthrone, but that only goes down to 100 ug/ml. 
Alterantively,I could do this by converting trehalose into 
glucose using trehalase. But again,commercial kits for glucose 
determination or published papers don't seem to reach this 
kind of sensitivity. I would like to avoid having to 
concentrate my samples by freeze drying.

Does anybody have any suggestions? 





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