Better method for deglycosylation...?

kzhao at BIOVX1.BIOLOGY.UCLA.EDU kzhao at BIOVX1.BIOLOGY.UCLA.EDU
Tue Aug 27 13:54:33 EST 1996


The trouble to deglycosylate plant glycoprotein by the conventional enzymatic
digestion (e.g., PNGase F) may be the fact that there is a unique Fuc1->3core
GlcNAc which almost blocks the activity of PNGase F.  I dont know if there 
is a 1,3fucosidase that can remove this sugar before you digest with PNGase F.

********************************************************************************
* Ke-Wei Zhao, Ph.D.                     Phone: (310)825-8722                  *
* Department of Biological Chemistry     Fax: (310)206-5272                    *
* UCLA School of Medicine, CHS 33-257    e-mail: kzhao at biovx1.biology.ucla.edu *
* Los Angeles, CA 90095-1737                                                   *
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