Better method for deglycosylation...?

WESTCM at COLLEGE.MED.UFL.EDU WESTCM at COLLEGE.MED.UFL.EDU
Wed Aug 28 12:23:06 EST 1996


>To:            glycosci at net.bio.net
>From:          kzhao at biovx1.biology.ucla.edu
>Subject:       Better method for deglycosylation...?
>Date:          27 Aug 1996 11:54:33 -0700
>
>
>The trouble to deglycosylate plant glycoprotein by the conventional 
>enzymatic
>digestion (e.g., PNGase F) may be the fact that there is a unique 
>Fuc1->3core
>GlcNAc which almost blocks the activity of PNGase F.  I dont know if there 
>is a 1,3fucosidase that can remove this sugar before you digest with PNGase 
>F.

TFMS digestion is likely to work although there may be other effects on the 
protein. Boehringer sells a PNGase A which cleaves a1,3-core fucosylated 
N-glycans. I don't know if it will cleave the difucosylated core. 

Chris West
Department of Anatomy & Cell Biology
University of Florida College of Medicine
Gainesville, Florida  32610-0235  USA
Ph 352-392-3329
Fax 352-392-3305
e-mail westcm at college.med.ufl.edu



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