inhibition of O-glycosylation

Olivier Nosjean nosjean at univ-lyon1.fr
Wed Dec 11 15:29:39 EST 1996


In article (Dans l'article) <32AEF369.6AF9 at ensam.inra.fr>,
urbach at ENSAM.INRA.FR (urbach) wrote (écrivait) :

> Hello all,
> 
> I try to determine if a protein is glycosylated using SF9 expression 
> system.
> I test tunycamicine effect on N-glycosylation.
> To test if "my" protein is o-glysosylated, I would like to use an 
> specific inhibitor, like neuraminidase. 
> ut is it a good inhibitor?, 
> Witch concentration have i to use?

Haem... neuraminidase is not a metabolic inhibitor, it is an enzyme that
hydrolyses the terminal non-reducing neuraminic acid residues (sialic acid
to make it short).

Alternatively, you can use Benzyl N-acetyl-alpha-D-galactosamide, a
competitive inhibitor of N-acetyl-alpha-D-galactosaminyl transferase,
which catalyses the first step in the biosynthesis of most O-linked
glycans.

The product is available through Oxford Glycosystems (distributed by COGER
in France : fax= 01.45.32.71.04)


> Excuse for my question, and my english but i am not a specialist.

There is no problem with that, as long as we can communicate !

> Thank you for answer directly to my e.mail adress

C'est parti ! Avec une reponse en anglais pour le newsgroup, ca peut
toujours servir...

> Serge URBACH
> urbach at ensam.inra.fr
> Laboratoire de Biochimie et physiologie moleculaire des plantes
> ENSAM/INRA/CNRS/UNII
> Montpellier

...Olivier

_  _  _
 \/ \/
  {O}               Olivier Nosjean
  |_|     Laboratoire de Physico-Chime Biologique
  |||           Univ. C. Bernard - Lyon 1  
   |                    France




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