inhibition of O-glycosylation

urbach urbach at ENSAM.INRA.FR
Wed Dec 11 03:44:27 EST 1996


Hello all,

I try to determine if a protein is glycosylated using SF9 expression 
system.
I test tunycamicine effect on N-glycosylation.
To test if "my" protein is o-glysosylated, I would like to use an 
specific inhibitor, like neuraminidase. But is it a good inhibitor?, 
Witch concentration have i to use?
Excuse for my question, and my english but i am not a specialist.
Thank you for answer directly to my e.mail adress

Serge URBACH
urbach at ensam.inra.fr
Laboratoire de Biochimie et physiologie moleculaire des plantes
ENSAM/INRA/CNRS/UNII
Montpellier



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