Digest using Pronase

kzhao at BIOVX1.BIOLOGY.UCLA.EDU kzhao at BIOVX1.BIOLOGY.UCLA.EDU
Mon Feb 12 13:11:22 EST 1996


For MS analysis (at least using MALDI-TOF), there is no need to desialylate
the glycoprotein, though sialic acid containing glycopeptide may have some
problem for detection.  I have successfully determined bi-antennary
complex glycopeptide with 0, 1, and 2 sialic acids based on Con A binding (
10 mM alpha-methylglucoside elution), sialidase and galactosidase digestions,
and MALDI-TOF analysis.

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*Ke-Wei Zhao, Ph.D.
*Dept. of Biological Chemistry
*UCLA School of Medicine
*Los Angeles, CA 90095-1737
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