Different behaviour of glycoprotein in two detection systems.
spagnol at GALACTICA.IT
Fri Jan 5 15:40:15 EST 1996
Dear fellow researchers,
A glycoprotein we exctrated from brains reacts in immunoblot with a
monoclonal against its peptide moyety, as a 100 kD band.
Following 2D SDS-PAGE, however, the single band becomes two spots, same
MW but PI at 4.4 and 5.1, respectively. We think this might e due to
carbohydrate eterogeneicity and we will perform treatment syalidase
treatment, as soon as we get the enzyme. Apart from this we had had no
Ideas and we also have no reference on how to study such cases, so that
any suggestion would be greatly welcome.
Thanks in advance for your corteous attention.
Giorgio Spagnol, MD
Institute of Neurology,
Statal University, Milan,
Via F. Sforza 35, 20122 Milan, Italy.
Phone: 02-55190390 W, 02-6570326 H
FAX: 02- 55190392
E-MAIL: spagnol at galactica.it
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