Different behaviour of glycoprotein in two detection systems.

[Giorgio Spagnol]

Dear fellow researchers,
A glycoprotein we exctrated from brains reacts in immunoblot with a 
monoclonal against its peptide moyety, as a 100 kD band.
Following 2D SDS-PAGE, however, the single band becomes two spots, same 
MW but PI at 4.4 and 5.1, respectively. We think this might e due to 
carbohydrate eterogeneicity and we will perform treatment syalidase 
treatment, as soon as we get the enzyme. Apart from this we had had no 
Ideas and we also have no reference on how to study such cases, so that 
any suggestion would be greatly welcome. 
Thanks in advance for your corteous attention.

Giorgio Spagnol, MD
Institute of Neurology,
Statal University, Milan,
Via F. Sforza 35,  20122 Milan, Italy.

Phone: 02-55190390 W, 02-6570326 H
FAX:    02- 55190392
E-MAIL: spagnol at galactica.it