Succinyl wheat germ

Irina Mathov irina at
Mon Jan 22 11:03:44 EST 1996

>From irina Fri, 19 Jan 96 15:47:14 ARG remote from idehu
>Received: by (UUPC/PcCorreo 3.0) with UUCP; Fri, 19 Jan 96 15:47:14 ARG
>Date: Fri, 19 Jan 96 15:47:14 ARG
>From: Irina Mathov <irina at>
>Message-ID: <672ao206 at>
>X-Mailer: UUPC/PcCorreo 3.0
>To: gycosci at
>Subject: Succinyl wheat germ
>Hi all
>I'm using biotinilated succinyl wheat germ to develope some western blots
>as Pierce indicates in the instructions but so far I have doubtfull results.
>When I use glycoproteins known to have terminal N Acetyl glucosamine the bands
>are very weak (I have tried different conc. of SWG)
>I couldn't find any references about the needs of cations in the solutions
>used for incubation (as for ConA).
>Does anyone know if SWG needs any cation to improve its binding?
>Any idea will be wellcome.
>Thanks a lot
>Irina Mathov

					Irina Mathov
					irina at

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