Sulfation of glycoprotein
spagnol at GALACTICA.IT
Sat Jan 27 17:53:41 EST 1996
Dear fellow researchers,
The glycoprotein I'm studiyng presents in vivo a carbohydrate epitope
composed of sulfated glucuronic acid, which make it detectable by
circulating IgM in some patients with neurophaty. The hypotesis is that
sulfatation is critical for antibody recognition
I transfected a cell line with the cDNA and verified that the
recombinant protein is recognized by the antibodies. Now I would like to
see if the recombinant protein contains S04.
I would be very grateful if someone could suggest a method and/or a
reference to do it.
Thanks in advance
Giorgio Spagnol, MD
Institute of Neurology,
Statal University, Milan,
Via F. Sforza 35, 20122 Milan, Italy.
Phone: 02-55190390 W, 02-6570326 H
FAX: 02- 55190392
E-MAIL: spagnol at galactica.it
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