Carbohydrate detection on Westerns

phillip buckhaults buckhaults at bscr.uga.edu
Sun Mar 3 03:34:50 EST 1996


Roy Jefferis wrote:
> 
> I would like to hear of experiences of detecting glycosylation of
> proteins on Western blots. We have had variable experiences with
> labelled lectins in detecting complex oligosaccharides of IgG
> antibody molecules. In the present instance we want to determine
> the presence or absence of oligosaccharide in the Fab fragments
> followingregion of mini-digests of paraproteins. If a commercial
> product has been found satisfactory please give source and details.
> Thanks, Roy Jefferis, Dept. Immunology.


I use biotinylated lectins from Vector (415-697-3600), and a SA-HRP 
conjugate with the ECL reagents from amersham.  works fine for detecting 
GlcNAcb1,6Man branches and polylactosamine on total cellular 
glycoproteins. good luck!



-- 
GlcNAcTransferase-V  GlcNAcTransferase-V  GlcNAcTransferase-V  
GlcNAcTransferase-V  

Phillip Joe Buckhaults
Department of Biochemistry and Molecular Biology and
Complex Carbohydrate Research Center
Life Science Building, Room B316
University  of  Georgia
Athens Georgia  30602
Phone (706) 542-1701
Fax     (706) 542-1759

"Let us not confuse effort with achievement"
                -Bill Buckhaults



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