Microplate assay for carbohydrates

[Shantha Raju]

Raymond McGuirk wrote as:

Dear Glycoscientists,

I am looking for a simple, sensitive and reproducable microplate 
assay for free or bound hexoses and/or sialic acids. I am trying to 
quantify the amount of carbohydrate being removed from a glycoprotein 
with endo- and exo- glycosidase enzymes over time. References would 
be much appreciated.

Thanking you in advance,
Raymond McGuirk
Department of Biochemistry,
University College Dublin,
Belfield,
Dublin 4, Ireland.
E-Mail:- McGuirk at ollamh.ucd.ie
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In Genentech, this is one of the aspect we are looking into. We are
presenting a poster on this subject at the Boston Meeting on
Glycobiology during the end of this month. If you have access to
interenet, you can look into our abstract. I would like to invite
Glycofolks to comment on this.
Thanks
T. Shantha Raju Ph.D.
 




-- 
Analytical Chemistry MS62
Genentech Inc.
460 Point San Bruno Blud.
South San Francisco, CA 94080
Tel. (415)225-8893
Fax. (415)225-3554
email: sraju at gene.com