We have an antibody (MUD50) that recognises Ser/Thr-O-GlcNAc (beta comformation) in
the context of a PTVT repeat. It will recognise a single T-GlcNAc and it also
recognises S-GlcNAc, but I am unsure whether the there are other amino acid sequence
constraints on the antibody recognition, eg it may require adjacent proline residues.
Thus, the lack of a reaction with MUD50 might be due to the particular amino acid
sequence. We would be eager to find out.
Cheers, Martin Slade
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>To: glycosci at net.bio.net>Subject: O-linked sugars
>From: (k.c.breen at dundee.ac.uk)
>Date: 19 Sep 1996 14:38:32 GMT
>>I would like to check for the presence of Ser/Thr-O-GlcNAc on a protein.
>Looking through the appropiate catalogues, I cannot find a lectin that
>specifically labels this sugar group. I would be grateful if somebody could
>give me details of how I may assay for this particular sugar linkage.
>>Thanks,
>>Kieran Breen, Dept. of Pharmacology,
>University of Dundee,
>Ninewells Hospital & Medical School,
>Dundee DD1 9SY,Scotland, U.K.
>>k.c.breen at dundee.ac.uk>>---------------------------------------
Martin Slade,
School of Biological Sciences,
Macquarie University,
NSW 2109,
Australia
FAX (61 2) 850 8174
Phone(61 2) 850 8210
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