known ConA binding proteins

[Corne Stroop]

Craig Webster wrote:
> 
> In article <jdb-230996164547 at wintermute.bioc.rice.edu>, John Bishop
> I use con A to bind glycoforms of alpha-1-acid glycoprotein (orosomucoid) in
> my work. As far as I know con A will bind to any protein that has bi-
> antennary glycans attached as this is the glycan motif that con A recognises.

Well, 'binding' should be replaced by 'retarded'. As David Ashford
already pointed out: ConA binds to oligomannose and hybrid type glycans.
So, using ConA can result in three (!) pools. A unretarded pool of
proteins bearing no or only tri/tetra-antennery glycans, a retarded pool
of glycoproteins bearing bi-antennary glycans, and a bound pool of
glycoproteins with oligomannose or hybrid type structures.

Yours Sincerely,

Corné

_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/CJMS_/_/_/
 Corné JM Stroop
 Department of Bio-Organic Chemistry
 Bijvoet Center for Biomolecular Research
 Padualaan 8
 NL-3584 CH  Utrecht, Netherlands
    PHONE: 31-30-2533752    FAX: 31-30-2540980
        E-MAIL: cjms at ruucj1.chem.ruu.nl
 
http://www.boc.chem.ruu.nl/~cjms/CSHP.html
_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/CJMS_/_/_/