shkim at COMP.KBSI.RE.KR
Mon Jun 30 03:48:28 EST 1997
I have questions about glycoprotein staining. I have partial purified
some protein with con-A sepharose. When I electrophoresed the partial
purified eluate, several major and minor bands ranged from 10 to 150 kD
were appeared on silver stained gel. But I didn't get any signal from
DIG detection on nitrocellulose membrane under about 50 kD. Of course,
standard glycoprotein, fetuin was stained well. So, I am wondering
whether all of the proteins eluted from lectin chromatography were
glycoprotein or a part of the protein was non-glycoprotein.
My second question is what product for glycoprotein staining is more
sensitive and specific. Any idea will be helpful to me.
Korea Basic Science Institute
shkim at comp.kbsi.re.kr
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