Maximum binding of proteinA-fusion protein to IgG agarose

Jun-Ichi Aikawa jaikawa at UCSD.EDU
Wed Oct 15 22:15:27 EST 1997

Dear glycobiologists,

Does anyone know the condition for maximum binding of proteinA-fusion
protein to IgG agarose? When I used a spent medium of transfected cells as
a source for the purification, I failed to purify it. Does it need to
adjust pH to the optimal condition for binding to IgG agarose?

Welcome any comments and suggestions.

Jun-ichi Aikawa, Ph.D.
University of California, San Diego

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