Total carbohydrate content

Ian McFarlane I.McFarlane at icrf.icnet.uk
Tue Oct 28 09:45:36 EST 1997


I've used this assay as well. I did the assay in plastic LP4 tubes (they
are polycarbonate I think), they melt a bit but don't seem to affect the
reaction. The most important thing we have found is to squirt the conc.
sulphuric directly onto the sample or the colour development isn't very
strong.

HTH Ian Mc

 
> >I am to measure total carbohydrate content in cereal
> >grain/flour. Would you suggest me a simple metod for such 
> >analysis. 
> >        Thanks in advance 
> 
> >        Rafal
> 
> I find the phenol/sulphuric assay to be pretty robust and sensitive, but it 
> does use some rather nasty reagents. You will need glass cuvettes and, 
> preferably, an old spectrophotometer. The method is in Dubois et al. (1956)
> Analytical Chemistry, v28, pp350-356.
> Jonathan Depree,
> Lincoln University, P.O. Box 84, Canterbury, New Zealand.
> 
> Socrates was a famous Greek Teacher who went around giving
> people advice. They killed him.   (school history howler)



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