firths at cryptic.rch.unimelb.edu.au
Wed Apr 22 19:19:09 EST 1998
Hi, I'm after protocols for the deglycosylation of whole cell proteins
from a mammalian cell pellet. I'm currently using PNGase F from NEB but on
whole cell proteins I am getting substantial degradation of protein.
Any suggestions would be welcome.
Trace Element Laboratory
The Murdoch Institute for Research into Birth Defects
The Royal Children's Hospital
More information about the Glycosci