Deglycosylation protocols

Steven Firth firths at cryptic.rch.unimelb.edu.au
Wed Apr 22 19:19:09 EST 1998


Hi, I'm after protocols for the deglycosylation of whole cell proteins
from a mammalian cell pellet. I'm currently using PNGase F from NEB but on
whole cell proteins I am getting substantial degradation of protein.
Any suggestions would be welcome.

Stephen Firth
Trace Element Laboratory
The Murdoch Institute for Research into Birth Defects
The Royal Children's Hospital
Melbourne, Australia.



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