Glucose Assay

Allen Bush bush at CHEM.UMBC.EDU
Sun Aug 30 07:18:13 EST 1998


Electrochemical methods work well in that concentration range.  These are
widely used in some HPLC systems and have been described in papers by D.
Johnson and W. Lacourse.
 On Fri, 28 Aug 1998, David A. Priestman wrote:

> Looking for a good reliable assay (preferably spectrophotometric or
> fluorescent) for glucose in the range >100pmol <5nmol.  Standard serum
> assays (hexokinase/G6PDH, Trinder etc) are too insensitive, fluorescent
> (2-AB) labeling difficult to separate free label from labelled glucose. 
> I would also like to be able to assay galactose in this range.
> 	Any suggestions would be greatly appreciated.
> 
> PS I know about luminometric methods, but don't have a luminometer
> immediately at hand.
> 

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Prof. C. Allen Bush, Dept. of Chemistry, Univ. Maryland Baltimore County,
1000 Hilltop Circle; Baltimore, MD 21250.  e-mail: bush at umbc.edu 
 website: http://www.research.umbc.edu/~bush/
phone: (410) 455-2506       fax: (410) 455-2608
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