Glucose Assay

Depree, Jonathan A depreej at lincoln.ac.nz
Mon Aug 31 10:42:26 EST 1998


In article <35E6F90E.6F55 at glycob.ox.ac.uk> "David A. Priestman" <priesty at glycob.ox.ac.uk> writes:
>From: "David A. Priestman" <priesty at glycob.ox.ac.uk>
>Subject: Glucose Assay
>Date: Fri, 28 Aug 1998 17:38:40 -0100

>Looking for a good reliable assay (preferably spectrophotometric or
>fluorescent) for glucose in the range >100pmol <5nmol.  Standard serum
>assays (hexokinase/G6PDH, Trinder etc) are too insensitive, fluorescent
>(2-AB) labeling difficult to separate free label from labelled glucose. 
>I would also like to be able to assay galactose in this range.
>        Any suggestions would be greatly appreciated.

>PS I know about luminometric methods, but don't have a luminometer
>immediately at hand.

Is there any reason you can't freeze-dry your samples and make up in a smaller 
amount of liquid?
Jonathan Depree,
Lincoln University, P.O. Box 84, Canterbury, New Zealand.

Socrates was a famous Greek Teacher who went around giving
people advice. They killed him.   (school history howler)



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