glycosidase

[Zhao, Kewei]

Fritz at "Muthgasse at net.bio.net" wrote:

Does anybody out there know which alpha-glucosidase is suitable for
de-glucosylation of Glc(n)Man(9) ?  It seems to be a rare task.

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According to Kornfeld & Kornfeld (Ann Rev. Biochem. 54:631-64, 1985),
alpha1,2-glucosidase I cleaves the outmost glucose residue, and alpha1,3-
glucosidase II cleaves the two inner glucose residues. 

Ke-Wei Zhao
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*Ke-Wei Zhao, Ph.D.                    Phone: (310)825-8722                  *
*Department of Biological Chemistry    Fax: (310)206-5272                    *
*UCLA School of Medicine, CHS 33-257   e-mail: kewei at biochem.medsch.ucla.edu *
*Los Angeles, CA 90095-1737                                                  *
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