Galactosidase protocol request

Andrew Woods awoods at dunn1.path.ox.ac.uk
Wed Jun 10 03:40:52 EST 1998


Hi,

I'm investigating a lymphocyte cell surface molecule and would like to
investigate the effect upon a number of epitopes by the removal of
galactosyl.

So, if anyone can supplyt me with a protocol to this end, suitable for
treating normal lymphocytes (and still having the cells intact
afterwards for flow cytometry), that would be a great help.

Thanks in advance

Andrew Woods.




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