Deglycosylation - Edge et al. (1981)

B Lauder R.Lauder at lancaster.ac.uk
Tue Oct 13 10:36:41 EST 1998


Hi,

Questions about de-glycosylation occur at least once a year here so not to 
disappoint you here is one.

Regarding the method of Edge et al. (1981) Anal. Biochem. 118; 131-137, 
who used TFMS to deglycosylate, does anybody have any data which 
point to protein degradation or reduced deglycosylation efficiency when 
following their methods. If so are these special cases requiring an 
alternative protocol?

On a more general note is anybody working on a hot new method for high 
efficiency broad spectrum deglycosylation which leaves the protein core 
intact?

Thanks,

Bob
-- 
Biological Sciences, Lancaster University, UK # 
R.Lauder at Lancaster.ac.uk




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