IUBio

Detection of proteoglycans and glycosaminoglycans

georgeyip at WRITEME.COM georgeyip at WRITEME.COM
Sat May 8 16:07:35 EST 1999


Hi!

I am writing to ask if anyone is familiar with the "critical electrolyte concentration" phenomenon in the detection of different glycosaminoglycans using alcian blue solution on paraffin-embedded sections.

The problem I have is that the alcian blue precipitates out of solution immediately upon the addition of magnesium chloride.

Any advice would be most deeply appreciated.

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