long-term degradation of enzyme with sephadex G-50

Eric Raskas eraskas at micro-sense.com
Mon Feb 7 22:52:55 EST 2000


I am trying to use Sephadex G-50 as a support for enzyme immobilization.  I
am finding that the enzyme's performance is degrading after a relatively
short period of time when mixed in a suspension with the Sephadex.  Can
anyone provide insight for me into this problem.  Is there some processing
required for the Sephadex.  The enzyme is a well understood flavin oxidase
enzyme.
I would very much appreciate your help.

Eric Raskas
MicroSense
St. Louis, Missouri
314 862-2320
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