3-year Post-doc York, UK

David Ashford da5 at york.ac.uk
Mon Feb 12 08:40:00 EST 2001


Department of Biology, University of York, UK

3-year Post-doctoral Research Post

The role of peptide:N-glycanases and N-glycans in plant development.

Funding is available for three years to appoint a postdoctoral research
assistant to carry out a multidisciplinary project to investigate the
functions of plant peptide:N-glycanases. The project will be carried out

in the laboratory of Dr. Michael Roberts, in collaboration with
Professor Dianna Bowles and Dr. David Ashford, combining relevant
experience in plant molecular biology, plant cell signalling,
glycoprotein biology and glycan analysis.

We have previously characterised a novel peptide:N-glycanase (PNGase)
activity which is developmentally regulated during fruit ripening in
tomato.  Work in other laboratories has also indicated that free
N-glycans, the products of PNGase activity, may be important signalling
molecules in plants.  We have now identified genes encoding PNGase
enzymes in both Arabidopsis and tomato, opening the way for a detailed
investigation of the role of PNGases and their products in plant
biology.

The major objectives in this project will be:

* to use transgenic plants to examine in detail the link between PNGase
enzyme activity and gene expression, free N-glycan levels, and the
programme of fruit development in tomato.

* to employ functional genomics to investigate broader roles for PNGase
activity in Arabidopsis through the analysis of PNGase gene expression
and the characterisation of PNGase knockout mutants.

* to assess the biochemical properties of recombinant PNGase enzymes
produced in the yeast Pichia pastoris.

The successful candidate must have a strong background in plant
molecular biology. In particular, experience of molecular genetics,
working with Arabidopsis and plant transformation, will be desirable.
Experience in plant biochemistry, biochemical analysis, protein
purification or recombinant protein production would also be beneficial.

Starting salary will be in the range of £16,775-£18,731, depending on
experience.

For further details contact Dr Michael Roberts (mrr5 at york.ac.uk) or Dr
David Ashford (da5 at york.ac.uk).




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    Dr David A. Ashford
    Glycobiology: Research & Analytical, Department of Biology,
    University of York, P.O. Box 373, York,  YO10 5YW  UK
    Tel: +44 (0)1904 434391           Fax: +44 (0)1904 434312
    Email: da5 at york.ac.uk
    WWW: http://www.york.ac.uk/depts/biol/staff/daa/daa.htm
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