de-glycosylation

DOCTORHIM at aol.com DOCTORHIM at aol.com
Sat Oct 27 16:58:37 EST 2001


The best way is to use a Deglycosylation kit.   The best and cheapest is made 
by Prozyme(www.prozyme.com).  There is a three enzyme kit for $250 and a five 
enzyme kit for $350.  It takes off both N and O-linked sugars. You can 
download a PDF version of the kit booklet.  Enough for at least 2 mgs of 
glycoprotein.  The distributor in the UK is Europa Bioproducts LTD.  You can 
find their phone number on the Prozyme web page.   Hope this helps.  

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