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<DIV>Eric,</DIV>
<DIV> </DIV>
<DIV>Is the flavin covalently linked to your enzyme? </DIV>
<DIV>Are there any other cofactors the enzyme might need, metal ions, etc.</DIV>
<DIV> </DIV>
<DIV>If not, try adding some flavin or the other factors to your immobilized
protein.</DIV>
<DIV> </DIV>
<DIV>Cheers,</DIV>
<DIV> </DIV>
<DIV>Achim</DIV>
<BLOCKQUOTE
style="BORDER-LEFT: #000000 2px solid; MARGIN-LEFT: 5px; MARGIN-RIGHT: 0px; PADDING-LEFT: 5px; PADDING-RIGHT: 0px">
<DIV>"Eric Raskas" <<A
href="mailto:eraskas@micro-sense.com">eraskas@micro-sense.com</A>> wrote in
message <A
href="news:000d01bf71e7$f7bce8c0$1400000a@eric">news:000d01bf71e7$f7bce8c0$1400000a@eric</A>...</DIV>
<DIV><FONT face=Arial size=2><SPAN class=450004803-08022000>I am trying to use
Sephadex G-50 as a support for enzyme immobilization. I am finding that
the enzyme's performance is degrading after a relatively short period of time
when mixed in a suspension with the Sephadex. Can anyone provide insight
for me into this problem. Is there some processing required for the
Sephadex. The enzyme is a well understood flavin oxidase enzyme.
</SPAN></FONT></DIV>
<DIV><FONT face=Arial size=2><SPAN class=450004803-08022000>I would very much
appreciate your help.</SPAN></FONT></DIV>
<DIV><FONT face=Arial size=2><SPAN
class=450004803-08022000></SPAN></FONT> </DIV>
<DIV><FONT face=Arial size=2><SPAN class=450004803-08022000>Eric
Raskas</SPAN></FONT></DIV>
<DIV><FONT face=Arial size=2><SPAN
class=450004803-08022000>MicroSense</SPAN></FONT></DIV>
<DIV><FONT face=Arial size=2><SPAN class=450004803-08022000>St. Louis,
Missouri</SPAN></FONT><FONT face=Arial size=2><SPAN
class=450004803-08022000></SPAN></FONT></DIV>
<DIV><FONT face=Arial size=2><SPAN class=450004803-08022000>314
862-2320</SPAN></FONT></DIV></BLOCKQUOTE></BODY></HTML>
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