<pre><font size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'>I’ll appreciate receiving information regarding the sialidase inhibitor 2,3 dehydro N- acetyl neuraminic acid:<o:p></o:p></span></font></pre><pre><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'><o:p> </o:p></span></font></pre>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>1. Is it being used in industry as a supplement to culture
medium in order to avoid cleavage of sialic acid residues of recombinant
glycoproteins expressed in CHO cells?<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>2. Same for protein free medium and CHO cells grown in
suspension.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>3. What is the recommended concentration to be used for the
above objective?<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>4. What is the level of purity of the product?<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>5. Analytical methods to detect traces of the inhibitor.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Thanks for your kind response,<o:p></o:p></span></font></p>