In article <1992Dec13.140531.1 at icrf.icnet.uk> p_travers at icrf.icnet.uk writes:
>Does anyone have an H2-Kd cDNA clone that they would be willing to send me?
>We are interested in peptide binding to MHC alleles and it turns out that Kd
>is best suited for our purposes
>> Paul Travers
>> Dept of Crystallography
> Birkbeck College, London
>P_TRAVERS at ICRF.ICNET.UK>
Below I have included the headers of three genes in Genbank which you
may find to be of interest - I would suggest that you look up the references
given here and contact the authors as they may well have the clone that you're
looking for. If you would like to retrieve the full entries (ie these headers
and the sequence) you can do so via Gopher or e-mail server - if you don't
know how to do either drop me a note and I'll send you instructions.
If you look up the references and come up with a location for but not
the address of the authors - post a note to bionet.users.addresses ( or the
bionaut e-mail address) and the group will try and find an address for you.
Best of luck,
Dan Jacobson
danj at welchgate.welch.jhu.edu
============================================================================
LOCUS MUSMHKD 1560 bp ss-mRNA ROD 07-NOV-1984
DEFINITION Mouse MHC class I H2-K gene (haplotype d), mRNA complete coding
sequence, clone pH-2-d-33.
ACCESSION J00402
KEYWORDS antigen; histocompatibility antigen.
SOURCE mouse (mus musculus), cDNA to mRNA, clone pH-2-d-33.
ORGANISM Mus musculus
Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 1560)
AUTHORS Lalanne,J.-L., Delarbre,C., Gachelin,G. and Kourilsky,P.
TITLE a cdna clone containing the entire coding sequence of a mouse h-2kd
histocompatibility antigen
JOURNAL Nucleic Acids Res. 11, 1567-1577 (1983)
STANDARD simple staff_review
COMMENT submitted as clean copy from authors.
FEATURES Location/Qualifiers
CDS 26..1131
/note="MHC H2-K antigen"
/codon_start=1
//
LOCUS MUSMHKDA 5141 bp ds-DNA ROD 16-JUN-1986
DEFINITION Mouse MHC class I H2-K gene (haplotype d), complete cds.
ACCESSION X01815
KEYWORDS antigen; class I antigen; histocompatibility antigen;
membrane protein; polymorphism.
SOURCE Mouse (DBA/2) liver DNA, clone lambda-2.14.
ORGANISM Mus musculus
Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 5141)
AUTHORS Kvist,S., Roberts,L. and Dobberstein,B.
TITLE Mouse histocompatibility genes: Structure and organisation of a K-d
gene
JOURNAL EMBO J. 2, 245-254 (1983)
STANDARD simple staff_review
COMMENT EMBL features not translated to GenBank features:
key from to description
PRM 160 164 CAAT-box
PRM 187 190 TATA-box
SITE 4460 4465 pot. polyA signal
SITE 4475 4480 pot. polyA signal
//
LOCUS MUSMHKDM 1111 bp ss-mRNA ROD 07-NOV-1984
DEFINITION Mouse MHC class I H2-K gene (haplotype d), mRNA, complete coding
sequence, clone pH-2-d-24.
ACCESSION K01182
KEYWORDS antigen; cell surface glycoprotein; class I antigen; glycoprotein;
histocompatibility antigen; major histocompatibility complex.
SOURCE mouse (DBA/2 (H-2d haplotype)) cDNA to liver mRNA, clone pH-2d-24.
ORGANISM Mus musculus
Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 1111)
AUTHORS Lalanne,J.-L., Cochet,M., Kummer,A.-M., Gachelin,G. and
Kourilsky,P.
TITLE Different exon-intron organization at the 5' part of a mouse class
I gene is used to generate a novel H-2Kd-related mRNA
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 80, 7561-7565 (1983)
STANDARD full staff_review
COMMENT Clone pH-2d-24 codes for 24 polypeptide, which is almost identical
to a genuine H-2Kd histocompatibility antigen. Clone pH-2d-24 was
compared with clone pH-2d-33, also sequenced by the authors, and
was found to be almost identical except at its 5' end. The authors
suggest that this gene may encode two distinct products through an
alternative use of splicing sites in the 5' region of the primary
gene transcript.
//
