On 16 Dec 1992, M.D. O'Leary wrote:
> Date: 16 Dec 92 15:24:53 GMT
> From: M.D. O'Leary <Mark.OLeary at newcastle.ac.uk>
> To: immunology at net.bio.net> Subject: ER - resident proteins
>> Hi,
>> I am trying to visualise ER-resident proteins (B1P etc) on an SDS-PAGE
> immunologically. I have tried lysed mammalian (hybridoma) cells, but got no
> detectable signal.
>> If anyone who has tried to do something similar, or has any suggestions
> (such as a method to enhance ER-resident protein levels in the applied sample?)
> I would be most grateful if they would contact me on mdo4 at le.ac.uk.>> PS. If anyone had another antibody raised against an ER-resident protein, that
> would also be useful to me as a control...
>> I hope someone will be able to help me...
>> Mark
>I don't have experiance with your specific problem (ER proteins), but
perhaps if
you could tell us a little more about your experimental conditions and
reagents, I (or someone else) could help pinpoint the problem, or at
least offer suggestions.
