Hybridoma problems

[Gregory Dandulakis]

In article <1992Jul21.162530.298 at bio.aau.dk> brian at bio.aau.dk writes:
>
>BUT!!! Then we passaged the hybridomas, and after this they are no longer
>able to proliferate.
>
>WHY?  has anyone expierence with the same thing, and especially can anyone
>give any suggestions, which might help us overcome this problem.


 There can be thousands of possible reasons. 

 The most common ones are:

                            1. Enough FBS?

                            2. Very low initial cell concentration?

 Give us some more feedback.


 Gregory Dandulakis
 Biochemical Engineering Lab
 U. of Virginia