IgM aggreg(v)ation

FGARBRECHT at mis.mcw.edu FGARBRECHT at mis.mcw.edu
Tue Sep 22 12:44:01 EST 1992


I am growing an IgM producing murine hybridoma in a small amicon bioreactor
under serum free conditions.  The IgM seems to be aggregating in the
reactor, to the best of my limited ability to tell (2 closely spaced
peaks on a size exclusion column).  Does anyone have a good,
inexpensive (ha) method to monitor antibody aggregation. (It takes
me 24 hours to run 1 sample over the column).  Any suggestions on how
to inhibit aggregates from forming?

Thanks,
Fred Garbrecht
Medical College of Wisconsin



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