Leukocyte Marker absent in platelets

bhjelle at carina.unm.edu bhjelle at carina.unm.edu
Wed Sep 23 12:29:46 EST 1992


In article <1992Sep23.153708.7433 at magnus.acs.ohio-state.edu> gchacko at magnus.acs.ohio-state.edu (George W Chacko) writes:
>
>I am performing a series of RT-PCR reactions on RNA from human
>platelets. In order to asuure myself that my platelet preps aren't
>contaminated with RNA from leukocytes I am looking for a molecule
>expressed on monocytes, lymphocytes and PMN's that is not expressed in
>platelets that I could use as a negative control. I intend to design
>PCR primers to that molecule so low levels of expression will also be
>picked up. I might have to use several sets of primers for each cell
>type but a single molecule would be ideal. Any suggestions?
>
Platelets express only class I MHC and not class II. That makes
them unique among hematopoeitic cells. You might wish to try
a class II MHC primer pair.

Brian



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