I have, over the past couple of years, gotten a
number of murine hybridomas to grow well in various
serum free media, but have been unsuccessful in
cryopreserving serum-free adapted hybrid cell lines.
I use the standard 10%DMSO/90%FCS as a cryopreservative,
which works fine for most of our cell lines, including
hybridomas grown in conventional serum containing
culture medium. However, the same lines, when adapted
to serum free culture seem to lose their cryopreservability.
I have not experimented much with this problem, and
since I am sure I'm not the only person facing this
problem, I was curious to hear about the experiance of
others; particularly with reference to using alternative
cryopreservatives like glycerol/DMSO in various combinations,
and the use of controlled rate freezing. Any comments
appreciated. Thanks in advance.
Fred Garbrecht
Medical College of Wisconsin
fred at bmt.mcw.edu
