markmano at markmano at
Mon Dec 13 18:40:28 EST 1993

Thank you all for your replies on my question about phage 
I am enclosing a summary of the responses I have got so far:

From:	IN%"ajgeorge at" 13-DEC-1993 11:49:45.99
To:	IN%"markmano at"
Subj:	phAge display

Ofer Markman

I enclose a few references re phage display systems. Hope they are of use.
I do not know how to send messages to immunology bulletin board via Gopher
so am sending direct to you.Any help on that much appreciated!

The references are fairly eclectic, I have concentrated on non-antibody
ones as they are less well known!

Phage display systems are marketed by Pharmacia (for antibodies) and
Promega. (there may be others).

Barbas, C. F., III, Kang, A. S., Lerner, R. A. & Benkovic, S. J. (1991).
Assembly of Combinatorial Antibody Libraries on Phage Surfaces: The Gene
III Site. Proceedings of the National Academy of Sciences (U.S.A.), 88,

Breitling, F., Dubel, S., Seehaus, T., Klewinghaus, I. & Little, M. (1991).
A Surface Expression Vector for Antibody Screening. Gene, 104, 147-153.

Clackson, T., Hoogenboom, H. R., Griffiths, A. D. & Winter, G. (1991).
Making Antibody Fragments Using Phage Display Libraries. Nature (London),
352, 624-628.

Cwirla, S. E., Peters, E. A., Barrett, R. W. & Dower, W. J. (1990).
Peptides on Phage: a Vast Library of Peptides for Identifying Ligands.
Proceedings of the National Academy of Sciences (U.S.A.), 87, 6378-6382.

Devlin, J. J., Panganiban, L. C. & Devlin, P. E. (1990). Random Peptide
Libraries: a Source of Specific Protein Binding Molecules. Science, 249,

George, A. J. T. (1993). The Production of Antibodies Using Phage Display
Libraries. In Monoclonal Antibodies, ed. M. A. Ritter, pp. in press.
Cambridge: Cambridge University Press. 

Greenwood, J., Willis, A. E. & R.N.Perham (1991). Multiple Display of
Foreign Peptides on a Filamentous Bacteriophage.  Peptides from Plasmodium
falciparum Circumsporozoite Protein as Antigens. Journal of Molecular
Biology, 220, 821-827.

Hammer, J., Takacs, B. & Sinigaglia, F. (1992). Identification of a Motif
for HLA-DR1 Binding Peptides Using M13 Display Libraries. Journal of
Experimental Medicine, 176, 1007-1013.

Hawkins, R. E., Russell, S. J. & Winter, G. (1992). Selection of Phage
Antibodies by Binding Affinity. Mimicking Affinity Maturation. Journal of
Molecular Biology, 226, 889-896.

Hoogenboom, H. R., Griffiths, A. D., Johnson, K. S., Chiswell, D. J.,
Hudson, P. & Winter, G. (1991). Multi-Subunit Proteins on the Surface of
Filamentous Phage: Methodologies for Displaying Antibody (Fab) Heavy and
Light Chains. Nucleic Acids Research, 19, 4133-4137.

Hoogenboom, H. R. & Winter, G. (1992). By-Passing Immunisation. Human
Antibodies from Synthetic Repertoires of Germline VH Gene Segments
Rearranged in vitro. Journal Molecular Biology, 227, 381-388.

Huse, W. D., Sastry, L., Iverson, S. A., Kang, A. S., Alting-Mees, M.,
Burton, D. R., Benkovic, S. J. & Lerner, R. A. (1989). Generation of a
Large Combinatorial Library of the Immunoglobulin Repertoire in Phage
Lambda. Science, 246, 1275-1281.

Kang, A. S., Barbas, C. F., Janda, K. D., Benkovic, S. J. & Lerner, R. A.
(1991). Linkage of Recognition and Replication Functions by Assembling
Combinatorial Antibody Fab Libraries along Phage Surfaces. Proceedings of
the National Academy of Sciences (U.S.A.), 88, 4363-4366.

Lowman, H. B., Bass, S. H., Simpson, N. & Wells, J. A. (1991). Selecting
High-Affinity Binding Proteins by Monovalent Phage Display. Biochemistry,
30, 10832-10838.

Marks, J. D., Hoogenboom, H. R., Bonnert, T. P., McCafferty, J., Griffiths,
A. D. & Winter, G. (1991). By-passing Immunization. Human Antibodies from
V-gene Libraries Displayed in Phage. Journal of Molecular Biology, 222,

Marks, J. D., Hoogenboom, H. R., Griffiths, A. D. & Winter, G. (1992).
Molecular Evolution of Proteins on Filamentous Phage. Mimicking the
Strategy of the Immune System. Journal of Biological Chemistry, 267,

Oldenburg, K. R., Longanathan, D., Goldstein, I. J., Scholtz, P. G. &
Gallop, M. A. (1992). Peptide Ligands for a Sugar-Binding Protein Isolated
from a Random Peptide Library. Proceedings of the National Academy of
Sciences (U.S.A.), 89, 

Roberts, B. L., Markland, W., Ley, A. C., Kent, R. B., White, D. W.,
Guterman, S. K. & Ladner, R. C. (1992). Directed Evolution of a Protein:
Selection of Potent Neutrophil Elastase Inhibitors Displayed on M13 Fusion
Phage. Proceedings of the National Academy of Sciences (U.S.A.), 89,

Schatz, P. J. (October 1993). Use of Peptide Libraries to Map the Substrate
Specificity of a Peptide-Modifying Enzyme - A 13 Residue Consensus Peptide
Specifies Biotinylation in Escherichia coli. Bio - Technology, 11,

Smith, G. P. (1985). Filamentous Fusion Phage: Novel Expression Vectors
that Display Cloned Antigens on the Virion Surface. Science, 228,

Stephen, C. W. & Lane, D. P. (1992). Mutant Conformation of p53. Precise
Epitope Mapping Using a Filamentous Phage Epitope Library. Journal of
Molecular Biology, 225, 577-583.

Tsunetsgu-Yokota, Y., Tatsumi, M., Robert, V., Devaux, C., Spire, B.,
Chermann, J. C. & Hirsch, I. (1991). Expression of an Immunogenic Region of
HIV by a Filamentous Bacteriophage Vector. Gene, 99, 261-265.

Good luck



Andrew J.T. George
Department of Immunology
Royal Postgraduate Medical School
Hammersmith Hospital
Du Cane Road
London W12 0NN

Tel 081 743 2030 ext 2089       From overseas +44 81 743 2030 ext 2089
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From:	IN%"J.Hexham at"  "Mark Hexham]" 13-DEC-1993 07:58:09.47
To:	IN%"markmano at"

There are several systems around for doing this for a reveiw and lots
of examples see Gene vol 23 I think where the whole issue is devoted
to applications of this method. The two vectors I know of for this
technique for antibodies are from Greg Winters lab sold as akit by
Pharmacia (in UK anyway) and the pComb 3 vector available from Carlos
Barbas or Dennis Burton at Scripps free for research purposes.

Date: Sat, 11 Dec 1993 15:09:47 -0500
From: Angelo Gunasekera <angelo at phoenix.Princeton.EDU>
Organization: Princeton University

Pharmacia sells a kit for peptide library work (specially designed to
make single chain antibodies)

Hope this helps


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