OLAF at UVVM.UVIC.CA
Mon Jun 28 17:14:38 EST 1993
Hello out there to all immunology netters...
I am trying to incorporate peptide antigens into distearoylphosphatidylcholi
ine/cholesterol unilamellar liposomes for immunization purposes. However, the
protocol I have for making dehydration/rehydration vesicles (Kahl et al, J. Imm
unol. 142:4441, 1989) doesn't seem to give me any incorporation. Furthermore,
the product apparently wont lyse in the presence of detergents, including 5% SD
S. Obviously I'm thinking that I'm doing something wrong, so I was wondering i
f anyone out there had a simple protocol for incorporating peptides into liposo
mes with this composition.
Thanks for any help you can provide.
L. Anthony, Ph.D.
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